Ca2+-Independent Release of Glutamate During In Vitro Anoxia in Isolated Nerve Terminals |
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Authors: | Inmaculada Rubio Magdalena Torres María Teresa Miras-Portugal Jose Sánchez-Prieto |
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Institution: | Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. |
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Abstract: | The effects of in vitro anoxia on the release of glutamate in isolated nerve terminals were studied. The extra-synaptosomal concentration of glutamate (Glu]ext) under aerobic conditions was 2.3 microM and increased to 4.9 microM after 10 min of anoxia. However, when synaptosomes were incubated in the presence of lactate plus pyruvate instead of glucose, to prevent anaerobic glycolysis, anoxia induced an eightfold increase in the Glu]ext. The accumulation of glutamate in the external medium during anoxia was Ca2+ independent and insensitive to a significant reduction of the Ca(2+)-dependent release of the amino acid. These results indicate that a Ca(2+)-independent efflux of cytoplasmic glutamate occurs during in vitro anoxia in isolated nerve terminals. |
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Keywords: | Glutamate release Ca2+-independent release-Vesicular release Anoxia Nerve terminals |
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