DNA of Akodon (Rodentia,Cricetidae). I. Biophysical characterization |
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Authors: | L Vidal-Rioja L Semorile N O Bianchi |
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Institution: | (1) Departmento de Citogenética Molecular, IMBICE, C. C. 403, 1900 La Plata, Argentina |
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Abstract: | Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699–1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The T
m
values determined in 1 × SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2–43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.This study was supported by grants from Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, and Organización de Estados Americanos. |
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Keywords: | Akodon Cricetidae DNA characterization |
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