Confocal laser scanning microscopy of fluorescently stained wood cells: a new method for three-dimensional imaging of xylem elements |
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Authors: | Werner Knebel Eberhard Schnepf |
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Affiliation: | (1) Zellenlehre, Fakultät für Biologie, Universität Heidelberg, Im Neuenheimer Feld 230, W-6900 Heidelberg, Federal Republic of Germany |
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Abstract: | Summary Xylem cells were fluorescently stained with periodic acid — Schiff reaction or with Schiffs reagent alone and studied by confocal laser scanning microscopy. Single images with extremely low depth of focus, series of optical sections, computed stereo scopic images and shadow casting images as well as x-z images are obtained. In contrast to scanning electron microscopy, not only are the surfaces imaged, but elements concealed by other structures can be visualized by this system. Quantitative data on cell depth are provided and differences in lignification are detected. |
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Keywords: | Confocal laser scanning microscopy Wood cell structure Three-dimensional imaging Lignification |
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