Effect of storage on amnesic shellfish poisoning (ASP) toxins in king scallops (Pecten maximus) |
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Authors: | EA Smith EP Papapanagiotou NA Brown LA Stobo S Gallacher AM Shanks |
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Institution: | Fisheries Research Services Marine Laboratory, P.O. Box 101, 375 Victoria Road, Aberdeen AB11 9DB, UK |
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Abstract: | Since 1998, king scallops (Pecten maximus) obtained from Scottish offshore sites have been monitored for domoic acid (DA) and epi-domoic acid (epi-DA), the principal toxic compounds associated with amnesic shellfish poisoning (ASP). The presence of these toxins in king scallops harvested from Scottish waters at concentrations exceeding the current regulatory limit (20 μg g−1 shellfish flesh) is a recurrent event. However, little information was available to determine the effects that different storage conditions experienced during sample transportation to the monitoring laboratory may have on the toxin concentrations, which are subsequently detected. Furthermore, the stability of DA and epi-DA in the solvents (methanol:water (1:1, v/v) and citric acid buffer (0.5 M, pH 3.2)) routinely used for their extraction from shellfish has not previously been assessed. Results from this study demonstrate that when king scallop samples were stored for 2–3 days at 12 °C, a significantly higher toxin concentration was detected in the gonad than when samples were stored at 4 °C and analysed within 48 h. This implies that monitoring programmes must consider transport and storage conditions between harvest and analysis. Stability studies showed rapid decomposition of DA and epi-DA in aqueous methanol extracts while DA and epi-DA seem acceptably stable when stored refrigerated in citrate buffer. |
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Keywords: | Amnesic shellfish poisoning (ASP) toxins Domoic acid (DA) epi-Domoic acid (epi-DA) Marine biotoxins Shellfish monitoring Sample storage |
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