Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase |
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Authors: | Marie E Maradeo |
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Institution: | Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA |
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Abstract: | Ctf7/Eco1-dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag-induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf7/eco1 mutant cells, suggesting that Smc1 and Smc3 head domains are in direct contact with each other and also with Mcd1/Scc1. Thus, cohesin complexes may be much more compact than commonly portrayed. We further demonstrate that mutation in ELG1 or RFC5 anti-establishment genes suppress tag-induced lethality, consistent with the notion that the replication fork regulates Ctf7/Eco1. |
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Keywords: | Cohesin Sister chromatids RFC complexes CTF7/ECO1 MCD1/SCC1 SMC1 |
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