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Predicted bacteriorhodopsin from Exiguobacterium sibiricum is a functional proton pump |
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| Authors: | LE Petrovskaya EP Lukashev SV Sychev EA Kryukova EV Spirina RA Khatypov DA Gilichinsky MP Kirpichnikov |
| Institution: | a Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russian Federation b Biological Department, Lomonosov Moscow State University, Moscow 119991, Russian Federation c Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russian Federation d Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russian Federation |
| Abstract: | The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 534 nm without dark adaptation. The ESR photocycle is characterized by fast formation of an M intermediate and the presence of a significant amount of an O intermediate. Proteoliposomes with ESR incorporated transport protons in an outward direction leading to medium acidification. Proton uptake at the cytoplasmic surface of these organelles precedes proton release and coincides with M decay/O rise of the ESR. |
| Keywords: | BR bacteriorhodopsin from Halobacterium salinarum ESR bacteriorhodopsin from Exiguobacterium sibiricum DDM d-maltopyranoside" target="_blank">n-dodecyl-β-d-maltopyranoside OG d-glucopyranoside" target="_blank">n-octyl-β-d-glucopyranoside |
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