|
|||||
|
|
Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells |
|
| Authors: | Shahhoseini Maryam Favaedi Raha Baharvand Hossein Sharma Vikram Stunnenberg Hendrik G |
| Affiliation: | a Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran b Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran c Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran d Radboud University, Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands |
| Abstract: | The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant. |
| Keywords: | RA, retinoic acid ChIP, chromatin immunoprecipitation ECC, embryonal carcinoma cell qRT-PCR, quantitative real time-polymerase chain reaction ACTB, beta-actin SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis FT-MS, Fourier transform mass spectrometry |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |