A mammalian insulysin homolog is regulated by enzyme IIA(Glc) of the glucose transport system in Vibrio vulnificus |
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Authors: | Kim You-Jin Ryu Yangkyun Koo Byoung-Mo Lee Na Yeon Chun Se-Jin Park Soon-Jung Lee Kyu-Ho Seok Yeong-Jae |
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Affiliation: | a Department of Biophysics and Chemical Biology and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea b Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea c Department of Environmental Science, Hankuk University of Foreign Studies, Yongin, Kyunggi-Do 449-791, Republic of Korea |
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Abstract: | Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus PTS. Here we show that enzyme IIAGlc of the PTS regulates the peptidase activity of a mammalian insulysin homolog in V. vulnificus. While interaction of IIAGlc with the insulysin homolog is independent of the phosphorylation state of IIAGlc, only unphosphorylated IIAGlc activates the insulysin homolog. Taken together, our results suggest that the V. vulnificus insulysin-IIAGlc complex plays a role in survival in the host by sensing glucose.Structured summaryMINT-8045996: IIA glu (uniprotkb:Q7MBY2) binds (MI:0407) to vIDE (uniprotkb:Q7MIS6) by pull down (MI:0096)MINT-8045817, MINT-8045967: IIA glu (uniprotkb:Q7MBY2) physically interacts (MI:0915) with vIDE (uniprotkb:Q7MIS6) by pull down (MI:0096) |
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Keywords: | PEP, phosphoenolpyruvate PTS, phosphoenolpyruvate:sugar phosphotransferase system EI, enzyme I of the PTS HPr, histidine-containing phosphocarrier protein IIAGlc, glucose-specific enzyme IIA of the PTS TFA, trifluoroacetic acid HPLC, high performance liquid chromatography vIDE, Vibrio vulnificus insulin-degrading enzyme EDTA, ethylenediaminetetraacetic acid EGTA, ethylene glycol tetraacetic acid NEM, N-ethylmaleimide TPEN, N,N,N&prime ,N&prime -tetrakis(2-pyridylmethyl)ethylenediamine MALDI-TOF, matrix-assisted laser desorption/ionization-time of flight PBS, phosphate-buffered saline TCBS agar, thiosulfate citrate bile salts sucrose agar |
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