Osmoregulated trehalose-derived oligosaccharides in Sinorhizobium meliloti |
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Authors: | Arnaud Brique Serge Pilard Isabelle Gosselin |
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Institution: | a Unité Génie Enzymatique et Cellulaire, UMR CNRS 6022, Université de Picardie Jules Verne, Amiens, France b Plate-Forme Analytique, Université de Picardie Jules Verne, Amiens, France |
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Abstract: | Sinorhizobium meliloti is a soil bacterium accumulating glutamate, N-acetylglutaminyl glutamine amide and trehalose in hyperosmolarity. Besides these compatible solutes, we highlighted several compounds in S. meliloti Rm1021 wild-type strain. The purification and the structural characterization based on liquid chromatography evaporative light scattering detector, electrospray ionization high resolution mass spectrometry and nuclear magnetic resonance techniques showed they were four linear oligosaccharides composed of 3, 4, 5 and 6 glucose units all linked by α-(1 → 2) linkages except a terminal α-(1 ↔ 1) linkage. These oligosaccharides were cytoplasmic and were observed in several wild-type strains suggesting they were common features in S. meliloti strains grown in hyperosmolarity. |
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Keywords: | DP Degree of polymerization ESI-HRMS Electrospray ionization high resolution mass spectrometry HMBC Heteronuclear multiple bond correlation LC-ELSD Liquid chromatography evaporative light scattering detector NAGGN N-acetylglutaminyl glutamine amide NMR nuclear magnetic resonance |
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