A Potential Epidemic Factor from the Bacteria, <Emphasis Type="Italic">Vibrio cholerae WO7</Emphasis> |
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Authors: | Shalmoli Bhattacharyya Jasleen Shant Nirmal K Ganguly Siddhartha Majumdar Sujata Ghosh |
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Institution: | (1) Department of Biophysics, Panjab University, Chandigarh, India;(2) Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India;(3) Indian Council of Medical Research, Government of India, New Delhi, India |
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Abstract: | Certain species of Vibrio cholerae have evolved mechanisms to become pathogenic to humans, with the potential to cause a severe life-threatening diarrheal disease,
cholera. Cholera can emerge as explosive outbreaks in the human population. V. cholerae illness is produced primarily through the expression of a potent toxin (cholera toxin) within the human intestine. The present
study has been carried out on a novel toxin purified from V. cholerae W07, an epidemic cholera strain devoid of cholera toxin gene (ctx). A modified method of purification improved purification fold as well as yield of this toxin. Heating was found to be the
essential and sufficient condition for dissociation of the two subunits (58 kDa and 40 kDa) of this toxin (pI 5.2). The 40-kDa
subunit of the purified toxin was identified as the carbohydrate binding subunit. This toxin was found to induce apoptosis
in HEp-2 cells. Thus, the WO7 toxin seems to have potential importance in the pathogenesis of disease associated with Vibrio cholerae WO7. |
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