Embryogenic cell lines of <Emphasis Type="Italic">Juniperus communis</Emphasis>; easy establishment and embryo maturation,limited germination |
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Authors: | Andreas Helmersson Sara von Arnold |
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Institution: | (1) Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O. Box 7080, 750 07 Uppsala, Sweden |
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Abstract: | Several coniferous species belonging to the Pinaceae family can be propagated via somatic embryogenesis, while species belonging to the Cupressaceae family cannot. The aim of this study was to identify possibilities and limitations with somatic embryogenesis in Cupressaceae. Juniperus communis was chosen as model species. We show that a high initiation frequency of embryogenic cell lines can be established from intact
megagametophytes at the time when intensive cleavage polyembryogeny takes place. The embryogenic cell lines proliferate fast
on medium lacking plant growth regulators. Early somatic embryos develop after transfer to medium with decreased content of
nitrogen and calcium. The early embryos mature after exposure to abscisic acid. Mature cotyledonary embryos germinate after
partial desiccation. A high proportion, over 40%, of the germinating embryos retain the embryogenic potential in the basal
part, resulting in development of new embryogenic tissue. |
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Keywords: | Cleavage polyembryony Cupressaceae Embryology Juniperus communis Somatic embryogenesis |
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