Characterization of sugar moieties and oligosaccharide sequences in the distal intestinal epithelium of the rainbow trout by means of lectin histochemistry |
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Authors: | B Pajak A Danguy |
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Institution: | Unitéde Morphologie fonctionnelle, Laboratoire de Biologic animale et Histologie compareée, CP 160114, UniversitéLibre de Bruxelles, 50 av. FD. Roosevelt, B-1050 Brussels, Belgium |
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Abstract: | Lectin histochemical study was performed on the distal intestinal mucosa of the rainbow trout. A panel of 25 biotinylated leetins for detection of sugar residues and oligosaccharide sequences of glycoconjugates on Bouin's solution-fixed paraffin embedded sections were used as probes. The avidin-biotin-peroxidase complex (ABC) was used to visualize the binding sites of lectins. A rich supply of carbohydrates was detected in this gut mucosal region. Marked differences in lectin labelling of goblet cells and enterocytes (absorptive cells) probably reflect various functional activities. The results are compared with those (scarce) obtained from other teleosts. Species differences in carbohydrate residue composition may be related to various feeding habits. The roles of posterior intestinal glycoconjugates in host-parasite interaction, macromolecular ingestion and immunological implications are discussed. |
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Keywords: | rainbow trout distal intestine lectin histochemistry |
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