Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase of Actinomadura R39. |
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| Authors: | C Piron-Fraipont C Duez A Matagne C Molitor J Dusart J M Frère and J M Ghuysen |
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| Institution: | Département de Microbiologie, Université de Liège, Sart Tilman, Belgium. |
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| Abstract: | By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39 beta-lactamase. Gene cloning resulted in an amplified expression of the beta-lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg.litre of culture-1). |
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