Agrobacterium-mediated transformation of Amaranthus hypochondriacus: light- and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter |
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Authors: | A E Jofre-Garfias N Villegas-Sepúlveda J L Cabrera-Ponce R M Adame-Alvarez L Herrera-Estrella J Simpson |
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Institution: | (1) Departamento de Ingenier′ιa Genética de Plantas, Unidad Irapuato (CINVESTAV-IPN), Apdo. Postal 629, 36500 Irapuato, Gto. México, XX |
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Abstract: | Mature embryos of Amaranthus hypochondriacus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cultivated
on Murashige and Skoog medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-2-methoxybenzoic acid and 10% coconut liquid endosperm. Transgenic plants
were obtained by inoculation of mature embryo explants with a disarmed Agrobacterium strain containing the plasmid pGV2260(pEsc4), which carried the genes encoding neomycin phosphotransferase type II and β-glucuronidase. The presence of transgenes in the genome of transformed amaranth plants and their progeny was demonstrated
by Southern blot hybridization. Tissue specific and light-inducible expression directed by a pea chlorophyll a/b-binding protein
promoter was observed in transgenic amaranth plants and their progeny.
Received: 30 December 1996 / Revision received: 14 May 1997 / Accepted: 3 June 1997 |
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Keywords: | Amaranthus hypochondriacus Agrobacterium tumefaciens NPT II Genetic transformation β -Glucuronidase Light regulation |
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