Effect of 2,4-dinitrophenol and ionophores on growth and methanogenesis inMethanobacterium thermoautotrophicum |
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Authors: | P ?migáň A Friederová P Rusňák M Greksák |
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Institution: | (1) Kosice, detached laboratory, Institute of Experimental Veterinary Medicine, 900 28 Ivanka pri Dunaji, Czechoslovakia;(2) Institute of Zoo-hygiene and Veterinary Technique, 918 21 Trnava |
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Abstract: | 2,4-Dinitrophenol and gramicidin D completely inhibited growth and methanogenesis inMethanobacterium thermoautotrophicum. At low K+ concentrations valinomycin inhibited growth and methanogenesis relatively slightly, at high K+ concentrations (0.1m KCl) growth was inhibited completely and methanogenesis by about 50%. Monensin and nigericin inhibited growth completely;
methanogenesis was inhibited like with valinomycin at high K+ concentrations. The results can be interpreted in terms of Mitchell’s chemiosmotic theory as follows. The protonmotive force
inM. thermoautotrophicum is the basic source of energy for endergonic processes. Dissipation of the electrical component of protonmotive force may
probably be compensated by an increased generation of the proton gradient. However, the osmotic component is essential for
growth ofM. thermoautotrophicum. |
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