Molecular monitoring of ruminal prevotellas |
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Authors: | Tepsic K Avgustin G |
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Institution: | (1) Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230 Domžale, Slovenia |
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Abstract: | The development and preliminary use of two different molecular approaches for rapid enumeration and monitoring of ruminal
prevotellas are described. Several oligonucleotide DNA probes, specific for the genusPrevotella and the speciesP. ruminicola andP. bryantii were labeled with various fluorochromes and used inin situ hybridization experiments. Epifluorescent microscopy was successfully used for the detection of fluorescent signal emitted
by the probes in pure and mixed culture samples. The enumeration of the target cells and the analysis of the crude rumen fluid
proved to be difficult, however, mainly due to the autofluorescent background and nonhomogeneous distribution of the cells
on the microscope slide. The development of a competitive PCR system for ruminal prevotellas is described and the preliminary
results of the rumen fluid analysis from a black-and-white Holstein cow are given.
An erratum to this article is available at . |
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