Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: role of xenobiotic metabolising enzymes and intestinal microflora |
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Authors: | Humblot Christèle Lhoste Evelyne Knasmüller Siegfried Gloux Karine Bruneau Aurélia Bensaada Martine Durao José Rabot Sylvie Andrieux Claude Kassie Fekadu |
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Affiliation: | Institut National de la Recherche Agronomique, Unité d'Ecologie et de Physiologie du Système Digestif, Batiment Jacques Poly, 78352 Jouy-en-Josas, France. |
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Abstract: | We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of beta-glucuronidase activity) following inulin consumption. |
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Keywords: | BCFA branched chain fatty acids CYP450 cytochrome P450 FU fluorescence units GST HAs heterocyclic amines HFA human flora associated IQ NAT SCFA short chain fatty acids SCGE single cell gel electrophoresis UDPGT UDP-glucuronosyl transferase XME xenobiotic metabolising enzymes Fermented milk Genotoxicity Oligosaccharides |
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