鼻咽癌组织的显微切割及其 RNA 线性扩增

从微小体积鼻咽癌活检标本中获取纯净癌细胞一直是鼻咽癌分子生物学研究中的难题 . 为了寻找一种能从鼻咽癌活检组织中获得高纯度、高质量 RNA 来完成 cDNA 微阵列 (cDNA Microarray) 实验的简便实用方法，采用 RNAlater 技术保存鼻咽癌活检组织，显微切割技术来获得高纯度鼻咽癌细胞，利用 RNA 线性扩增技术得到 cDNA 微阵列实验所需 RNA. 结果表明：利用 RNAlater 技术可以很好地保持组织 RNA 的稳定，通过优化显微切割和 RNA 线性扩增的条件获得了 cDNA 微阵列实验所需的高纯度、高质量 RNA.

Microdissection and RNA Line-amplification of Nasopharyngeal Carcinoma Tissue

It is a difficult problem in molecular biology study of nasopharyngeal carcinoma(NPC) to obtain pureness tumor cells from tiny NPC tissue.To seek a convenient and applied way to obtain pureness and high quality RNA from NPC tissue for cDNA Microarray,the study was designed to use RNAlater technology to keep the NPC dissected tissue and obtain pureness NPC cells by microdissection and gain RNA through RNA line amplification technology. The results hinted that RNAlater technology could keep the stability of NPC tissue and obtain the pureness and high quality RNA for cDNA Microarray combined with microdissection and RNA line amplification through optimum conditions.