一个小麦丝氨酸-苏氨酸蛋白激酶基因的克隆和分析 |
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引用本文: | 牛吉山,于玲,马正强,陈佩度,刘大钧.一个小麦丝氨酸-苏氨酸蛋白激酶基因的克隆和分析[J].Journal of Integrative Plant Biology,2002,44(3):325-328. |
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作者姓名: | 牛吉山 于玲 马正强 陈佩度 刘大钧 |
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作者单位: | 牛吉山(国家小麦工程技术研究中心,郑州 450002.);于玲,马正强,陈佩度,刘大钧(南京农业大学农业部作物细胞遗传重点实验室,南京,210095) |
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基金项目: | 国家高技术研究发展计划(863计划),教育部重点实验室基金 |
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摘 要: | 用mRNA差异显示技术在含有抗白粉病基因Pm21的小麦(Tri ticum aestivum L.) -簇毛麦(Haynaldia villosa) 6VS /6AL易位系92R137中分离与抗白粉病相关的基因,获得一个命名为TaPK1的全长cDNA克隆.序列分析表明,它与大豆(Glycine max (L.) Merr.)蛋白激酶基因GmPK6高度同源.经推测,TaPK1 编码416个氨基酸的多肽,属丝氨酸-苏氨酸蛋白激酶家族,并具酪氨酸激酶特性.TaPK1是从小麦中分离的新基因.
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关 键 词: | 小麦 白粉病 蛋白激酶 |
Molecular Cloning and Characterization of a Serine/Threonine Protein Kinase Gene from Triticum aestivum |
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Authors: | NIU Ji Shan YU Ling MA Zheng Qiang CHEN Pei Du LIU Da Jun |
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Abstract: | To isolate genes related to resistance to Erysiphe graminis DC. ex Merat f.sp. tritici Em. Marchal in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from the seedlings of the wheat Haynaldia villosa 6VS/6A L translocation line 92R137 that contains the powdery mildew resistance gene Pm21. A full length cDNA named TaPK1 was isolated. BLAST analysis revealed that it was significantly homologous to Glycine max (L.) Merr. protein kinase (GmPK6) cDNA. TaPK1 encodes a 416 amino acid long polypeptide, which belongs to serine/threonine protein kinase family, also has tyrosine kinase specificity. TaPK1 is a novel protein kinase from wheat. |
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Keywords: | wheat powdery mildew protein kinase |
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