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Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato |
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| Authors: | Mihály?Kondrák,János?Kutas,Borbála?Szenthe,András?Patthy,Zsófia?Bánfalvi,Miklós?Nádasy,László?Gráf,Bence?Asbóth author-information" > author-information__contact u-icon-before" > mailto:asboth@abc.hu" title=" asboth@abc.hu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
| Affiliation: | (1) Institute of Plant Biology, Agricultural Biotechnology Center, 2101 Gödöllo˝, P.O. Box 411, Hungary;(2) Entomological Laboratory of the Plant Protection, Institute of Georgikon Faculty of Agricultural Sciences of Veszprém University, 8360 Keszthely, Deák F. Str. 57, Hungary;(3) Department of Biochemistry, Eötvös Loránd University, 1117 Budapest, Pázmány Str. 1/C, Hungary |
| Abstract: | The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into potato by Agrobacterium-mediated transformation. From 23 independent transgenic lines, three with high mRNA level and proteinase inhibitory activity were propagated in vitro and transferred to pots. The peptide from the leaves was identified by its N-terminal sequence and by Ki values against chymotrypsin and trypsin. Colorado potato beetle larvae reared on transgenic plants grew slightly but significantly more slowly than those on control plants. This supports the notion that expression of multifunctional proteinase inhibitors of insect origin might be a good strategy to improve insect resistance in plants. |
| Keywords: | beetle resistance larval growth locust inhibitory peptide multifunctional inhibitor transgenic potato |
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