Salivary progesterone for the assessment of the ovarian function in the capuchin monkey (Cebus apella) |
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Authors: | L DiGiano C A Nagle S Quiroga N Paul Z Farinati M Torres A F Mendizabal |
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Institution: | (1) Centro de Investigación en Reproducción Humana y Experimental Centro de Educación Médica e Investigaciones Clínicas (CEMIC), Galván 4102, 1431 Buenos Aires, Argentina |
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Abstract: | Salivary and plasma progesterone were measured in normally cycling (n=10) and castrated (n=4) femaleCebus monkeys (Cebus apella). During the follicular phase, progesterone levels in saliva ranged between 0.05 and 1.40 ng/ml and in the luteal phase they
increased to between 0.22 and 4.70 ng/ml. These values represented on average 6.5 and 3.2% of those values measured in plasma,
for the follicular and luteal phases, respectively. The regression analysis of the steroid concentrations in both fluids showed
a highly significant correlation (r=0.8985,n=180,P<0.0001). Ovariectomized monkeys had consistently low salivary (0.37±0.02 ng/ml) and plasma (4.70±0.25 ng/ml) progesterone,
showing a low, but significnat, correlation coefficient (r=0.2592,n=58,P=0.047). The ratio of plasma/salivary progesterone was significantly higher in the luteal phase (31.09±1.65) than in the follicular
phase (23.06±2.26) and in castrated monkeys (16.00±1.38). The free fraction of progesterone constituted 5.3±0.2% of the total
plasma progesterone during the follicular phase and 3.3±0.1% during the luteal phase. Ovariectomized monkeys showed a significantly
higher percentage of free progesterone in plasma (7.7±0.1%). In contrast, free progesterone made up 64.4 and 70.9% of the
total salivary progesterone for the follicular and luteal phases, respectively. The proportion of free progesterone in castrated
animals was within the range observed in cycling animals. We suggest that the levels of progesterone in the saliva of capuchin
monkey follow a pattern similar to that for plasma progesterone, reflecting the free steroid fraction. Thus, the measurement
of such steroid in saliva may offer a valuable alternative to plasma determinations for the assessment of the ovarian function
inCebus and probably other New World monkey species. |
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Keywords: | Cebus apella saliva progesterone menstrual cycle ovariectomy |
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