Primary cultures of heart cells from the scallp Pecten maximus (mollusca-bivalvia) |
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Authors: | F Le Marrec-Croq D Glaise C Guguen-Guillouzo C Chesne A Guillouzo V Boulo G Dorange |
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Institution: | 1. Unité de Culture cellulaire, 6 avenue Le Gorgeu, Université de Bretagne Occidentale, BP 809, 29285, Brest Cedex, France 2. INSERM, U49, H?pital Pontchaillou, 35033, Rennes Cedex, France 3. Société BIOPREDIC, 14, rue J. Pecker, 35000, Rennes Cedex, France 4. INSERM, U456, Faculté de Pharmacie, 35043, Rennes Cedex, France 5. IFREMER/CNRS U.M.R. 219, Université de Montpellier 2, 2 Pl. E. Bataillon, BP 80, Montpellier Cedex, France
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Abstract: | Summary Primary cultures of Pecten maximus heart cells, isolated by an enzymatic procedure, were routinely obtained with a high level of reproducibility in a simple
medium based on sterile seawater. Cells attached to the plastic substratum without the need to add a special factor. The number
of adhering cells gradually increased with the time of culture. Two types of adhering cells were observed: epitheliallike
cells and fibroblastlike cells, which were more numerous. The latter cells were identified as myocytes by electron microscopy
and immunofluorescent staining. Results obtained by autoradiography, after incorporation of 14C]leucine, 3H]thymidine, and 14C]acetate, confirmed functional activity of the cells. These cultures were maintained viable in vitro during at least 1 mo. |
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Keywords: | Mollusca marine bivalve Pecten maximus scallop heart cell culture |
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