Plant regeneration of Solanum lycopersicoides Dun. from stem explants,callus and suspension cultures |
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Authors: | L W Handley K C Sink |
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Institution: | (1) Department of Horticulture, Michigan State University, 48824 East Lansing, MI, USA;(2) Present address: Westvaco Corporation, Forest Research, Box 1950, 29484 Summerville, SC, USA |
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Abstract: | Protocols were established for achieving plant regeneration from stem internode, callus, and cell suspension cultures of Solanum lycopersicoides Dun. Two accessions of S. lycopersicoides exhibited different responses as to callus formation on various media, requirement of gibberellic acid for shoot regeneration, and ability to grow in suspension culture. The optimum medium for initiation and maintenance of cell suspension cultures was Murashige and Skoog 9] medium with 15 mg l– NAA. For shoot regeneration, of three cytokinins tested, zeatin was found most effective relative to number, rapidity of response and overall quality of shoots. Shoot regeneration from stem explants, callus and suspension cultures was optimum on MS + 3.0 mg l–1 zeatin + 0.1 mg l–1 gibberellic acid.Michigan Agricultural Experiment Station Journal Article No. 11589. |
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Keywords: | Solanum lycopersicoides tomato callus suspension regeneration embryo-genesis gibberellic acid |
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