Isolation and characterization of microsatellite loci in Hexagrammos otakii based on 2b-RAD method |
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| Authors: | Zhen Shen Bo Wang Fawen Hu Hongzhan Liu Hongbin Guan Fengrong Zheng |
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| Institution: | 1. First Institute of Oceanography MNR, Qingdao, China;2. Marine Biology Institute of Shandong Priovince, Qingdao, China;3. Marine College of Shandong University, Weihai, China |
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| Abstract: | In this study, we isolated 20 novel microsatellites loci associated with the growth of Hexagrammos otakii (Fat greenling) by using 2b-RAD sequencing method. The characteristics of 20 microsatellite loci were amplified in 105 H. otakii individuals which came from four different groups and tested by Capillary electrophoresis. The number of allele of the 20 microsatellite loci ranged from 8 to 26 with an average of 19.95. PIC value ranged from 0.1659 to 0.9227 with an average of 0.7555. Observed and expected heterozygosity varied from 0.0667 to 0.8571 and from 0.1710 to 0.9314, respectively. Highly polymorphic characteristics were observed in each microsatellite loci of the study except HO6. Further research showed that 10 microsatellite loci had transferability in Hexagrammos agrammus. In this study, high polymorphism and genetic diversity using the 15 polymorphic microsatellite loci suggest that they are suitable for investigating the fine-scale population structure, genetic relationship and further evaluating the artificial reproduction strategy of H. otakii. |
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| Keywords: | Hexagrammos otakii mircrosatellite next gereration sequencing polymorphism |
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