Isolation and characterization of microsatellite loci in Pimelodus microstoma (Siluriformes: Pimelodidae) |
| |
| Authors: | Camila Satie Savada Moema Cristina Costa de Lima Mário Luís Orsi Fernanda Simões de Almeida |
| |
| Institution: | 1. Laboratório de Genética e Ecologia Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, Paraná, Brazil;2. Laboratório de Ecologia de Peixes e Invasões Biológicas, Departamento de Biologia Animal e Vegetal, Universidade Estadual de Londrina, Londrina, Paraná, Brazil |
| |
| Abstract: | Ten microsatellite loci were developed and described for Pimelodus microstoma, identified in eggs collected from the lower Das Cinzas river. Nine loci were polymorphic, with 02–19 alleles per locus. The observed heterozygosity values were up to 0.898. Five species of the Pimelodidae family were also tested for amplification of loci, among which Pimelodus maculatus was the most successful in the transferability of primers. These loci have proven to be useful for studying the variability and structuring of populations of Pimelodus microstoma. |
| |
| Keywords: | catfish freshwater neotropical SSR transferability |
|
|
