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Direct plant regeneration from cucumber embryonal axis |
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| Authors: | A Vasudevan N Selvaraj A Ganapathi C W Choi M Manickavasagam S Kasthurirengan |
| Institution: | (1) Department of Biology and Medicinal Science, Pai Chai University, Daejeon, 302735, South Korea;(2) Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu, India;(3) Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli, 620023 Tamil Nadu, India |
| Abstract: | Embryonal axis explants from 2-d-old in vitro germinated seeds were used to induce multiple shoot production. The combination of 4.44 μM BA and 1.59 μM NAA in MS medium triggered the initiation of adventitious shoot buds. The explants with shoot buds produced maximum number of shoots (10.6 per explant) in MS medium supplemented with 4.44 μM BA and 0.065 mM L-glutamine in three successive transfers. The elongated shoots were rooted on MS medium with 4.92 μM IBA. Rooted plants were transferred to soil with a survival rate of 65 %. |
| Keywords: | adventitious shoot bud benzyladenine Cucumis sativus L L-glutamine naphthalene acetic acid |
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