Induction and production of cellulases by l-sorbose in Trichoderma reesei |
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Authors: | Mikio Kawamori Yasushi Morikawa Seigo Takasawa |
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Institution: | (1) Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., Asahimachi, 194 Machida-Shi, Tokyo, Japan;(2) Research and Development Division, Kyowa Hakko Kogyo Co. Ltd., Otemachi, Chiyoda-ku, 100 Tokyo, Japan |
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Abstract: | Summary Cellulase production in Trichoderma reesei mutants was induced by l-sorbose, known to be an inhibitor of -1,3-glucan synthesis. In the experiments the washed mycelia were used as resting cells. For CMCase induction over 24 h using T. reesei PC-3-7, the most effective pH, temperature and l-sorbose concentration were 2.8, 28° C and 0.3 mg/ml, respectively. Comparison with other cellulase inducers showed that the inductive level of CMCase by l-sorbose was similar to that by sophorose, known to be the most potent inducer of cellulases. Since the induction of CMCase was inhibited completely by 10 g of cycloheximide per ml, the induction process was considered to involve de novo synthesis. Although l-sorbose had the effective inducibility of CMCase, the assimilation rate of l-sorbose was very low in T. reesei PC-3-7.Production of Ethanol from Biomasses. Part III.Production of Ethanol from Biomasses. Part III. |
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