结核分枝杆菌感染诱导的人巨噬细胞细胞因子及其调控基因的差异表达谱

研究人巨噬细胞细胞因子在抗结核分枝杆菌感染免疫中的作用。利用表达谱芯片技术研究细菌感染后，宿主巨噬细胞基因表达情况，在全局表达谱分析基础上，重点分析细胞因子及相关基因的表达，并比较无毒株和临床分离有毒株在诱导细胞因子及其调控基因表达方面的差异。结果显示细菌感染影响较多细胞因子及其调控基因的表达，如IFN、TNF、TGF、IL系列及其受体、NFkappa B和TLR受体等；首次报道IL19参与了抗结核分枝杆菌感染免疫。被临床株感染影响的细胞因子较无毒株广泛和丰富。细胞因子及相关基因参与了宿主细胞对感染细菌的免疫应答，有关细胞因子及相关基因在抗结核免疫中的作用有待进一步研究。

Differential Expression of Human Macrophage Genes Encoding Cytokines and Their Regulatory Elements After Mycobacterium tuberculosisInfection

To investigate whether the genes encoding cytokines and their regulatory element s participated in the immune response to \%Mycobacterium tuberculosis\% infectio n. Expression microarray was employed to compare the avirulent strain and clinic ally isolated strains infection induced macrophage c ytokine differential expression. Results were cytokines IFN, TNF,TGF, IL and the ir regulatory elements are involved in the immune reaction. IL-19 was first rep orted to be involved in the anti-Mtb immunity. Relative expression level of the ses factors before and after infection were assayed too.The data provides clues for further scrutinize the role of cytokines and related elements in the i nteraction between \%Mycobacterium tuberculosis\% and host macrophage.