Enzymic synthesis of o-succinylbenzoyl-CoA in cell-free extracts of anthraquinone producing Galium mollugo L. cell suspension cultures |
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| Authors: | Lutz Heide Rainer Kolkmann Sibylle Arendt Eckhard Leistner |
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| Affiliation: | (1) Institut für Pharmazeutische Biologie und Phytochemie der Westfälischen Wilhelms-Universität, Hittorfstraße 56, D-4400 Münster, F.R.G. |
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| Abstract: | o-Succinylbenzoic acid (OSB) is an intermediate in the biosynthesis of shikimatederived anthraquinones. The cell free activation of o-succinylbenzoic acid in extracts of anthraquinone producing cells of Galium mollugo L. is demonstrated for the first time. This activation depends on the presence of ATP, coenzyme A and Mg2+. The o-succinylbenzoic acid coenzyme A ester was identified by converting it to 1,4-dihydroxy-2-naphthoic acid by a bacterial enzyme, viz. naphthoatesynthase. It is thus demonstrated that the o-succinylbenzoic acid coenzyme A ester derived from bacteria and from Galium mollugo cells are identical. |
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