Direct actions of cortisol, thyroxine and growth hormone on IGF-I mRNA expression in sea bream hepatocytes |
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Authors: | Leung L Y Kwong Anna K Y Man Angel K Y Woo Norman Y S |
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Institution: | aDepartment of Biology, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China |
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Abstract: | The present study aims to investigate potential regulatory effect of different growth-related hormones including growth hormone (GH), human insulin-like growth factor-I (hIGF-I), thyroxine (T4), triiodothyronine (T3) and cortisol, on insulin-like growth factor-I (IGF-I) mRNA expression of hepatocytes isolated from silver sea bream. By using real-time PCR, IGF-I mRNA expression profiles of hepatocytes in response to individual hormones were determined in vitro. Hepatocytes incubated with GH at concentrations of 10–1000 ng/mL showed significantly higher IGF-I expression, but the elevation was attenuated at high concentration of GH (1000 ng/mL). IGF-I expression remained unchanged in hepatocytes after incubation with hIGF-I. Hepatocytes incubated with T4 at concentration of 1000 ng/mL exhibited a significant elevation in IGF-I expression, whereas no difference in IGF-I expression was demonstrated in hepatocytes after incubation with T3. Upon incubation with cortisol (1–1000 ng/mL), IGF-I expression was significantly decreased in hepatocytes in a dose-dependent manner. Our study demonstrated that GH, T4, and cortisol had direct modulatory effects on IGF-I expression in fish hepatocytes in vitro. |
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Keywords: | Primary hepatocyte culture Sea bream Hormones IGF-I mRNA expression profile Real-time PCR |
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