Adr亚型乙型肝炎病毒核心抗原基因的全顺序分析

采用定点克隆与随机克隆相结合的实验设计,用α~(35)S-dATP标记,以末端终止法测定了adr亚型乙型肝炎病毒DNA核心抗原基因的全顺序,包括终止密码子在内基因全长552核苷酸,编码由183氨基酸组成的多肽,计算分子量21000dlt。与日本及上海等实验室报道的adr亚型HBcAg基因比较,核苷酸点突变1.3—1.8％,由点突变导致的错义氨基酸突变有一处,占全部编码氨基酸的0.5％。与adw及ayw亚型比较,核苷酸点突变占全部核苷酸的9.8—10.9％,导致的错义突变占全部编码氨基酸的3.3—4.4％,并对eAg基因的定位进行了讨论。

ANALYSIS OF THE NUCLEOTlDE SEQUENCE OF CORE ANTIGEN GENE OF Adr SUBTYPE HEPATITIS B VIRUS

Using the shutgun strategy and cloning of DNA restriction fragments technology,the sequence of HBcAg gene,adr subtype,was determined by dideoxy method and labeled with a35S dATP as radioactive marker.The results indicated that the adr NC-1 core antigen gene is composed of 552 mucleotides from codon ATG to TAG and encoding the polypeptide of 183 amino acids with a molecular weight of 21000 dlt.In comparison with the data published in Japan and Shanghai,there are 1.3-1.8% nucleotides change between adrNC-1 and other adr subtypes corresponding to 0.5% missense mutation,and 9.8-10.9% nucleotides change between adrNC-1 and adw ayw with 3.3-4.4% missense mutation.