Effect of micellar lipids on rabbit intestinal brush-border membrane phospholipid bilayer integrity studied by31P NMR |
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Authors: | Claude Vallet-Strouve Charles Tellier Serge Poignant Philippe Boucrot |
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Affiliation: | (1) Centre de Recherche en Biologie et Physico-Chimie Cellulaires, U.A 472 CNRS-Faculte des Sciences, Universite de Nantes, 44072 Nantes-cedex, France |
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Abstract: | Summary The effect of biliary salts and fatty acids on the bilayer structure of rabbit intestinal brush-border membranes was studied using the nonperturbing probe31P NMR. The broad. asymmetric lineshape of the31P NMR spectrum of isolated brush-border vesicles demostrates that their component phospholipids are organized in extended bilayers. These membranes are not significantly perturbed by incubation with physiological concentrations of biliary salts (3, 9, 18mm), demonstrating that the vesicles are highly stable, corresponding to their biological function. However, the emergence of a narrow peak superimposed on the broad lineshape indicates that a small proportion of the membrane phospholipids has reached isotropic motion, which may correspond to external or internal micellar structures. Incubation with mixed micelles of fatty acids and taurochlorate show that long-chain fatty acids enhance the membrane-perturbing effect of taurocholate while short-chain, watersoluble fatty acids do not, suggesting a difference in the absorption mechanisms. |
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Keywords: | intestine brush border phospholipid biliary salt fatty acid 31P NMR |
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