Nonlinear kinetics of the xanthine oxidase reaction catalyzed by chicken liver xanthine dehydrogenase |
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| Authors: | D G Priest J R Fisher |
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| Affiliation: | Department of Biochemistry, Medical University of South Carolina, Charleston, South Carolina 29401 USA; Department of Chemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306 USA |
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| Abstract: | The xanthine oxidase reaction catalyzed by chicken liver xanthine dehydrogenase has been shown to give nonlinear kinetics of the type which has been identified as substrate activation. When a very wide range of substrate (pteridine) concentrations were studied, it was found that a downward deflection in reciprocal plots (substrate activation) occurs in the high region and an upward deflection in the very low region. When product (isoxanthopterin) was included in reaction mixtures, the upward deflection was enhanced and shifted to higher substrate concentration ranges. In addition, reciprocal plots with a second substrate (oxygen) and a product (isoxanthopterin) were nonlinear. |
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