Qualitative and Quantitative Analysis of Cyclic Nucleotides and Related Enzymes by Static and Dynamic Fast Atom Bombardment Mass Spectrometry |
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| Authors: | James I. Langridge A. Gareth Brenton Terry J. Walton Frank M. Harris Russell P. Newton |
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| Affiliation: | Biochemistry Group, School of Biological Sciences, and Mass Spectrometry Research Unit Department of Chemistry , University of Wales Swansea , Singleton Park, Swansea, SA2 8PP, United Kingdom |
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| Abstract: | Abstract Fast atom bombardment mass spectrometry in concert with collisionally-induced dissociation and mass-analysed kinetic energy spectrum scanning has enabled unequivocal identification of putative cyclic nucleotides in tissue extracts; quantitative analyses have enabled kinetic studies of cyclic nucleotide-related enzymes with multi-component monitoring, and dynamic spectrometric systems provide a potential means of continuous assay for these enzymes. |
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