Improved Procedure for the Reduction of N - 1 Content in Synthetic Oligonucleotides |
| |
Authors: | Radhakrishnan P. Iyer Dong Yu Zhiwei Jiang Sudhir Agrawal |
| |
Affiliation: | Hybridon Inc., One Innovation Drive , Worcester, Massachusetts, 01605 |
| |
Abstract: | Abstract By incorporating a “capping step” at the start of an oligonucleotide synthesis (“pre-cap”) and following a “SUP” work-up protocol with ammonium hydroxide, an overall improvement is observed in the quality of oligonucleotides synthesized on a large scale on controlled pore glass support (CPG). Rationalization of these results is provided. |
| |
Keywords: | |
|
|