Improved Procedure for the Reduction of N - 1 Content in Synthetic Oligonucleotides |
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| Authors: | Radhakrishnan P Iyer Dong Yu Zhiwei Jiang Sudhir Agrawal |
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| Institution: | Hybridon Inc., One Innovation Drive , Worcester, Massachusetts, 01605 |
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| Abstract: | Abstract By incorporating a “capping step” at the start of an oligonucleotide synthesis (“pre-cap”) and following a “SUP” work-up protocol with ammonium hydroxide, an overall improvement is observed in the quality of oligonucleotides synthesized on a large scale on controlled pore glass support (CPG). Rationalization of these results is provided. |
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