Interactions of Calf Spleen Purine Nucleoside Phosphorylase with Antiviral Acyclic Nucleoside Phosphonate Inhibitors: Kinetics and Emission Studies |
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| Authors: | J. Wierzchowski E. Kulikowska A. Bzowska A. Holy L. Magnowska D. Shugar |
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| Affiliation: | 1. Department of Organic Chemistry , Pedagogical University , 54 3-go Maja St., 08-111, Siedlce, Poland;2. Department of Biophysics , Institute of Experimental Physics, University of Warsaw , 93 Zwirki &3. Wigury St., 02 089, Warsaw, Poland;4. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic , Praha, Czech Republic |
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| Abstract: | Abstract Association between calf spleen purine nucleoside phosphorylase and a series of phosphonylalkoxyalkyl derivatives of purine bases was studied by inhibition kinetics and fluorimetric titrations. Dissociation constants, determined by fluorimetric titration in phosphate-free conditions, were lower than inhibition constants in 1 mM phosphate, and inhibition was still weaker in 50 mM phosphate, in accord with the postulated bisubstrate analogue character of this class of inhibitors. |
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