Oligonucleotides Quality Control Analysis in Free Solution by Capillary Electrophoresis at Acidic pH |
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| Authors: | Maria Flora Mangano Cristina Battaglia Giuliana Salani Luigi Rossi Bernardi Gianluca De Bellis |
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| Institution: | 1. Dipartimento di Scienze e Tecnologie Biomediche , Universita′ degli Studi di Milano;2. Istituto di Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche, L.I.T.A , Via Fratelli Cervi 93, 20090, Segrate, Italy |
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| Abstract: | Abstract A new method for fast, automated and inexpensive oligonucleotides analysis by capillary electrophoresis at low pH is presented. This method does not need any sieving media to resolve a mixture of polynucleotides which are analysed in free solution and separated on the base of composition and not length. This technique has been used to test a large set of standard and modified oligonucleotides thus to be applied in oligos routine quality control. |
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