How to Synthesize A tRNA? |
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| Authors: | W Pfleiderer K P Stengele F Bergmann M Resmini C Henke |
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| Institution: | Fakult?t für Chemie , Universit?t Konstanz , Postfach, 5560, D-78434, Konstanz |
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| Abstract: | Abstract New blocking group combinations have been investigated to achieve an automated synthesis of a tRNA and structural analogs on solid-support. The use of the 4-methoxytetrahydropyranyl group for 2′-OH-protection and the dansylethoxycarbonyl group for the 5′-OH position shows in the phosphoramidite approach good results. In the arabino series the 2-(4-nitrophenyl)ethoxycarbonyl group is a perfect 2′-OH blocking group which can be combined with the dimethoxytrityl residue in the usual manner to give high yields and pure materials. |
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