Covalent Attachment of Methylene Blue to Oligonucleotides |
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| Authors: | F. Schubert A. Knaf U. Möller D. Cech |
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| Affiliation: | Humboldt-Universit?t, Institut für Chemie , Hessische Str. 1–2, 10099, Berlin, Germany |
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| Abstract: | Abstract The synthesis and application of oligonucleotides derivatized by methylene blue are described. For that, a carboxylated methylene blue derivative was synthesized and transformed into an activated N-hydroxysuccinimidoester. The activated ester was reacted with 5′-aminoalkylated oligonucleotides. The labelled oligonucleotides were isolated and characterized both by reversed phase HPLC and MALDI mass spectrometry. Initial studies on analytical application of these oligonucleotide conjugates are discussed. |
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