Enhanced Cellular Uptake of G-Rich Oligonucleotides |
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| Authors: | A. Peyman A. Ryte M. Helsberg G. Kretzschmar M. Mag E. Uhlmann |
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| Affiliation: | Hoechst Aktiengesellschaft , G 838, D-65926, Frankfurt, am Main, Germany |
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| Abstract: | Abstract Sequence-dependency of cellular uptake of oligonucleotides into Vero cells has been studied. Cellular uptake of 5′-[35S]-labelled homopolymers decreased in the order (dG)16 >> (dT)16> (dA)16 > (dC)16. The change of two base-pairs (dG → dA) in a dG-rich antisense oligonucleotide with good antiviral activity dramatically decreased cellular uptake and abolished antiviral activity. |
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