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Reasons and Limits of Substrate Activity of Modified L-dNTP in DNA Biosynthesis |
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| Authors: | Alexander A Krayevsky Natalya B Dyatkina Dmitry G Semizarov Liubov S Victorova Elena A Shirokova Fritz Theil |
| Institution: | 1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences , 32 Vavilov str., Moscow, 117984, Russia;2. Centre of Selective Organic Synthesis , Rudower Chaussee 5, D-12484, Berlin-Adlershof, Germany |
| Abstract: | Abstract Theoretical and experimental analysis of interaction of modified D- and L- dNTP as substrates for template-dependent and template-independent DNA polymerases was performed. It is shown that if the modified nucleoside 5′-triphosphates do not contain a substituent in position 3′ DNA chains can be extended by both strereoisomeric series with the same kinetic parameters. But the presence of even a 3′- hydroxy group in L-dNTP prevents their incorporation into the DNA chain. |
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