Base Changes and Triple-Helix Hybridization Properties of GT Containing Third Strands: A Systematical Study |
| |
Authors: | Jean-Pierre Clarenc Bernard Lebleu Jean-Paul Léonetti |
| |
Affiliation: | 1. CNRS UMR9942 , Institut de Génétique Moléculaire du CNRS , PBP 5051, 34033, Montpellier, cedex;2. Université de Montpellier 2 Sciences et Techniques du Languedoc , PBP 5051, 34033, Montpellier, cedex |
| |
Abstract: | Abstract Triple-helix recognition of the DNA major grove offers a powerful approach for the design of sequence specific DNA binding molecules. Although the triple-helix binding of pyrimidine containing third strands is well characterized now, the factors governing the recognition of double stranded DNA by GT containing third strands are poorly understood. Oligonucleotides with a variable amount of G and GpT or TpG steps have been synthesized here. A systematical study was performed to determine the influence of these changes on the triple-helix hybridization properties of these oligonucleotides. Large difference in the hybridization properties have been observed by band shift experiments. |
| |
Keywords: | |
|
|