Uniform Band Intensities in Fluorescent Dye Terminator Sequencing |
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| Authors: | Shiv Kumar C. W. Fuller S. Nampalli M. Khot I. Livshin L. Sun |
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| Affiliation: | Amersham Pharmacia Biotech , 26111 Miles Road, Cleveland, OH, 44128, U.S.A. |
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| Abstract: | Abstract The use of Cyanine dye (CyTM 5 and Cy5.5) labeled dideoxy terminators with Thermo SequenaseTM DNA polymerase in DNA sequencing provides uniform band intensity, improved sequence read-length, and accuracy. It also greatly improves the ability to detect single base heterozygotes with dye-terminator sequencing method. |
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