Metallothionein mRNA expression in fetal mouse organs |
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Authors: | A J Ouellette |
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Institution: | 1. Cell Biology Unit, Shriners Burns Institute, Surgical Services, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;2. Department of Surgery, Harvard Medical School, Boston, Massachusetts 02114 USA;3. Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02114 USA |
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Abstract: | The regulation of metallothionein biosynthesis in mammalian development was investigated by examining organs of 17-day fetal mice for biologically active metallothionein mRNA. Metallothionein was identified in cell-free translation products by migration in polyacrylamide gels and its characteristic elution on Sephadex G-50 columns. Metallothionein constitutes ~7.5% of 35S]cysteine incorporated into polypeptides directed by mRNA from fetal liver, but it is not detectable in mRNA-directed products of fetal kidney, small bowel, heart, or adult liver. Consistent with a fetal-specific role, hepatic metallothionein mRNA content decreases abruptly in newborn mice, becoming undetectable within 12 days. |
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