The role of eucaryotic cell surface glycoconjugates in DNA replication |
| |
Authors: | Subhash Basu Prabir Bhattacharya Manju Basu |
| |
Institution: | 1.Department of Chemistry, Biochemistry, Biophysics and Molecular Biology Program,University of Notre Dame,Notre Dame,USA |
| |
Abstract: | Multiple forms of ricin have been isolated from castor bean seeds. Two forms, ricin-1 and ricin-2, differ in their isoelectric pI values and toxicity towards IMR-32 cells. Inhibition of IMR-32 DNA polymerase α2 is more pronounced with ricin-1 (65%) than with ricin-2 (10%). Ricin B chain (pI = 5.2) isolated from ricin-1 binds to IMR-32 cell surfaces as well as inhibits DNA polymerase α2 activity when studiedin vitro. The presence of galβ-linked glycoconjugates near the active site of IMR-32 DNA polymerase α2 has been proposed. Replication modulators which bind to the glycose portion of the enzymes involved in the replication system may need a mandatory binding to cell surface glycoconjugates for their activity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|