Human erythrocyte specific lectin from the seeds of Indian coral tree,<Emphasis Type="Italic">Erythrina variegata</Emphasis> Linn,var.<Emphasis Type="Italic">orientali</Emphasis> Linn,Merrill |
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Authors: | T K Datta P S Basu |
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Institution: | 1.Process Biochemistry Department,Indian Institute of Chemical Biology,Calcutta |
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Abstract: | Human erythrocyte specific lectin was isolated from the seeds ofErythrina variegata Linn. var.orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures. |
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