Fluorescence polarization as a tool to study lectin-sugar interaction |
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| Authors: | M Islam Khan M K Mathew T Mazumdar D Pain N Surolia M K Das P Balaram A Surolia |
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| Institution: | 1.Molecular Biophysics Unit,Indian Institute of Science,Bangalore;2.Department of Biochemistry Indian Institute of Science,Bangalore;3.Enzyme Engineering Laboratory,Indian Institute of Chemical Biology,Jadavpur, Calcutta |
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| Abstract: | The binding ofRicinus communis agglutinin andAbrus agglutinin to 4-methylumbelliferyl β-D-galactopyranoside was studied by equilibrium dialysis, fluo-rescence quenching and fluorescence polarization. The number of binding sites and the association constant value obtained by fluorescence polarization for bothRicinus communis agglutinin andAbrus agglutinin are in close agreement with those obtained by the other methods. This indicates the potential of ligand-fluorescence polarization measurements in the investigation of lectin-sugar interactions. |
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