Chromosomal location and copy number in wheat and some of its close relatives of genes for enzymes involved in photosynthesis |
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| Authors: | Shiaoman Chao Christine A Raines Marian Longstaff Peter J Sharp Michael D Gale and Tristan A Dyer |
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| Institution: | (1) Cambridge Laboratory, Institute of Plant Science Research, Maris Lane, Trumpington, CB2 2JB Cambridge, UK;(2) Present address: Department of Biology, University of Essex, Wivenhoe Park, CO4 3SQ Colchester, UK;(3) Present address: Sainsbury Laboratory, John Innes Institute, Colney Lane, NR4 7UH Norwich, UK |
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| Abstract: | Summary Homologous probes for the wheat coding sequences of the enzymes phosphoribulokinase, phosphoglycerate kinase (both chloroplast and cytosolic forms), chloroplast fructose-1,6-bisphosphatase and the small subunit of ribulose-1,5-bisphosphate carboxylase were used to determine the copy number and chromosomal location of the genes encoding these enzymes by restriction fragment length polymorphism analysis. Heterologous probes were similarly used to characterize the genes for the enzymes glyceraldehyde phosphate dehydrogenase (both chloroplast and cytosolic forms), phosphoenolpyruvate carboxylase and pyruvate, orthophosphate dikinase. Several of the genes are present in single copies per haploid genome, and the different enzymes are encoded by loci dispersed on different chromosomes. The significance of these findings is discussed in relation to gene expression and control of copy number. |
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| Keywords: | C4 photosynthesis cDNA probes Photosynthetic carbon reduction cycle enzymes Restriction fragment length polymorphism analysis |
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