Integration of differentiation signals during indirect flight muscle formation by a novel enhancer of Drosophila vestigial gene |
| |
Authors: | Frédéric Bernard Petar Kasherov |
| |
Institution: | Equipe Génétique Moléculaire de la Différenciation, Institut Jacques Monod, CNRS-Université Paris Diderot, Bâtiment.Buffon - 15 rue Hélène Brion, 75205 Paris CEDEX 13 - France |
| |
Abstract: | The gene vestigial (vg) plays a key role in indirect flight muscle (IFM) development. We show here that vg is controlled by the Notch anti-myogenic signaling pathway in myoblasts and is regulated by a novel 822 bp enhancer during IFM differentiation. Interestingly, this muscle enhancer is activated in developing fibers and in a small number of myoblasts before the fusion of myoblasts with the developing muscle fibers. Moreover, we show that this enhancer is activated by Drosophila Myocyte enhancing factor 2 (MEF2), Scalloped (SD) and VG but repressed by Twist, demonstrating a sensitivity to differentiation in vivo. In vitro experiments reveal that SD can directly bind this enhancer and MEF2 can physically interact with both SD and TWI. Cumulatively, our data reveal the interplay between different myogenic factors responsible for the expression of an enhancer activated during muscle differentiation. |
| |
Keywords: | Indirect flight muscles Vestigial Scalloped Twist Myocyte enhancing factor 2 Drosophila Muscle differentiation Myogenesis |
本文献已被 ScienceDirect 等数据库收录! |